• br Materials and methods br Author disclosure

  2018-10-24

  

  Materials and methods Author disclosure statement 5. Acknowledgments This work was supported by the UK Medical Research Council grants G0701172 and G0801061. We thank Dr. Yacoub Khalaf, director of the Assisted Conception Unit of Guy\'s and St. Thomas\' NHS Foundation Trust and his staff f

• Recently work has been focused on

  2018-10-24

  

  Recently, work has been focused on the adipose tissue-derived stromal cell (ASCs) population, since retrieval is straight forward and non-harmful coupled to low-risk surgical procedures. However, biological properties of ASCs have only been assessed by indiscriminate isolation from mostly white fat

• We have demonstrated that donor specific

  2018-10-24

  

  We have demonstrated that donor-specific DNAm patterns are maintained upon reprogramming into iPSCs (Shao et al., 2013), and these also remain upon redifferentiation into iPS-MSCs. Thus, there is some epigenetic memory after chromatin remodeling—whether these donor-specific DNAm patterns are functio

• bosentan A recent study indicated that XIST expression can b

  2018-10-24

  

  A recent study indicated that XIST expression can be lost upon prolonged passaging of female hiPSCs, referred to as “erosion of XCI” (Mekhoubad et al., 2012). This epigenetic erosion was found to be irreversible and correlated with a loss of differentiation characteristics and is very relevant for d

• Our selection free targeting approach enables rapid generati

  2018-10-24

  

  Our selection-free targeting approach enables rapid generation of knockin reporter lines, though it also requires either using established iCas9 SR 3576 or creating new iCas9 lines in a desired hPSC background. The upfront effort for generating iCas9 cells is relatively small due to the efficient T

• To establish whether replacement of lost podocytes by

  2018-10-24

  

  To establish whether replacement of lost podocytes by RPCs could influence CKD outcome, we traced podocyte regeneration to compare mice that underwent proteinuria remission with those that developed persistent proteinuria and CKD using two different lineage-tracing strategies. By tracking podocytes

• ESC CMs can be subdivided into atrial and

  2018-10-24

  

  ESC-CMs can be subdivided into atrial and ventricular CMs (Burridge et al., 2012). It has been documented that the expression of the α-isoform of myosin heavy chain (α-MHC), MLC2a, and MLC1a become restricted to the atrium during murine development (Zammit et al., 2000). Here, we employed MLC2a and

• Gpr or T cell death associated gene Tdag is

  2018-10-24

  

  Gpr65, or T cell death-associated gene 8 (Tdag8), is a proton/acid-activated GPCR (Choi et al., 1996; Wang et al., 2004). Whereas GPR65 had not been linked to HSPC transitions, it was reported to be a pro-apoptotic factor in glucocorticoid-induced lymphocyte apoptosis (Malone et al., 2004) and to re

• The definition of marker combinations in neural

  2018-10-24

  

  The definition of marker combinations in neural stem cell biology has been hampered by the dearth of information linking markers and the underlying lineage-defining biology. LPA is of particular interest as it suggests lipids as a new class of molecules co-defining stem cell entities, and indeed lip

• Another question is the heterogeneity within and between

  2018-10-24

  

  Another question is the heterogeneity within and between organoids, which seems common to all the protocols developed so far but has not yet been studied in detail. Several processes, such as progenitor proliferation, cell differentiation, and ontogenetic cell death, could be potential sources of or

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments We thank Ms. Y. Ogata for technical assistance. Financial support for this research was provided by the Uehara Memorial Foundation, Takeda Science Foundation, the Naito Foundation, the Ministry of Education, Culture, Sports, Scien

• purchase baicalein Epigenetic modification by active DNA dem

  2018-10-24

  

  Epigenetic modification by active DNA demethylation or histone demethylation has been shown to facilitate iPSC induction in human and mouse (Huangfu et al., 2008a, 2008b). Tet1, a DNA methylcytosine dioxygenase, can facilitate iPSC induction by promoting Oct4 demethylation and re-activation, and eve

• br Introduction Myelination of the CNS of

  2018-10-24

  

  Introduction Myelination of the CNS of mice occurs mainly within the first 4 post-natal weeks when oligodendrocyte progenitor cells (OPCs) differentiate into myelin-forming oligodendrocytes. After this period, adult OPCs of divergent developmental origins remain scattered throughout the CNS paren

• The goal of this study

  2018-10-24

  

  The goal of this study was to understand how synthetic hydrogels influence global biological function compared with standard 2D or 3D cell-culture formats. Vascular morphogenesis was chosen as a model biological system, and a bioinformatics approach was employed to provide genome-wide analysis for H

• pak4 br Experimental Procedures br Author Contributions br A

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments We thank Qian Yu for assistance with the induction of pluripotent stem cells and Dr. Bing Song for assistance with the flow cytometry assays. We thank Profs. Xiaofang Sun and Yong Fan of the Key Laboratory for Major Obstetric Dise

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