In our reprogramming culture aside from

2018-10-20

In our reprogramming culture, aside from insulin production, we also observed expression of α and δ cell hormones, glucagon and somatostatin, respectively in a sub-population of cells. This is likely due to the constitutive expression of reprogramming factors Ngn3 and Pax6. Both transcription factor

br Results br Discussion Extracellular

2018-10-20

Results Discussion Extracellular signaling factors play critical roles in regulating the balance between stem cell self-renewal and differentiation (Jones and Wagers, 2008; Spradling et al., 2001). In the present study, we identify CRIPTO as one such factor that promotes multipotency and maint

br Results br Discussion Our report represents a

2018-10-20

Results Discussion Our report represents a genome-wide study to understand the genetic variance in culture-expanded human MSCs. The emergence of multiple low-frequency SNCs in primary marrow MNCs (Figure 2A) indicates the existence of small clones carrying unique private somatic mutations, con

Pitolisant hydrochloride Our data confirm previously unrecog

2018-10-20

Our data confirm previously unrecognized levels of temporal diversity and complexity in the subclonal landscape of GBM. In all cases analyzed, genetically distinct subclones had variable serial repopulating activity in vivo. We can exclude the possibility that the presence of in vitro aberrations pl

Using a chemically defined monolayer neural differentiation

2018-10-20

Using a chemically defined, monolayer neural differentiation system (Lippmann et al., 2014), we determined that by temporally modulating Wnt/β-catenin and FGF signaling, hPSCs could be differentiated into pure SOX2+/Brachyury+ NMPs that were stable for 7 days. Over this period, the NMPs exhibited co

Surprisingly we found that the presence

2018-10-20

Surprisingly, we found that the presence of a single SNP 43 bp away from the PKLR TALEN cutting site was an impediment to HR. The presence of an SNP, which disrupts the complete matching between the genome sequence and homology arm, has already been reported to reduce the frequency of HR (Deyle et a

br Results br Discussion The cognitive

2018-10-20

Results Discussion The cognitive deficits associated with prenatal VPA exposure might not due solely to the reduced neurogenesis with the abnormal neuronal morphology in the hippocampus, and there is a possibility that the low freezing responses in fear associative tests were contributed by th

br Experimental Procedures br Author Contributions br Acknow

2018-10-20

Experimental Procedures Author Contributions Acknowledgments We thank Y. Sasai (RIKEN, Japan) for the RAX-GFP mESC line, M. Wegner (University of Erlangen, Germany) for SOX9, E. Tanaka (CRTD, Germany) for RAX and CRX, and D. Forrest (NIDDK, USA) for TRβ2 antibodies. We thank J. Brzezinski (

The adult mammalian kidney is

2018-10-20

The adult mammalian kidney is an organ with very low cell cycling during homeostasis but remarkable proliferating capacity after injury. It is still unresolved whether the kidney contains bona fide stem cells. Humphreys et al. (2008) genetically marked cells using Six2, a transcription factor expres

Levels of pro or anti inflammatory cytokines

2018-10-20

Levels of pro- or anti-inflammatory cytokines are not always predictive of the response, possibly due to the dynamic cytokine combinations (and concentrations) present in the cell microenvironment (Table S1). For example, relatively low inflammatory stimulus ( differentiation of cardiac fibroblasts

br Results br Discussion In this

2018-10-20

Results Discussion In this study we have shown that activating BRAF mutations leading to increased RAS/MAPK pathway signaling induce a hypertrophic phenotype in hiPSC-derived CMs. While BRAF-mutant CMs display intrinsic defects in Ca2+ handling, several aspects of their phenotype require parac

Hello world!

2018-07-29